Research in Hristov's lab focuses on sustainable dairy nutrition for efficient milk production.

Current research is in the areas of mitigation of nutrient losses and gaseous emissions from dairy operations and protein and amino acid nutrition of dairy cattle. Projects are exploring nutrition, feeds and diet formulation, feed additives, and natural and synthetic bioactive compounds to mitigate enteric methane production in dairy cows and enhance their immune function, improve nutrient utilization, gut and overall animal health, and increase milk production. Another area of research is focused on feeding low-protein diets to reduce nitrogen losses and ammonia emissions from manure while maintaining milk production and composition through maximizing microbial protein synthesis in the rumen and supplementing the diet with rumen-protected amino acids.