Research in Hristov's lab focuses on sustainable dairy nutrition for efficient milk production.

Projects are centered on feeding low-protein diets to reduce nitrogen losses and ammonia emissions from manure while maintaining milk production and composition through maximizing microbial protein synthesis in the rumen and supplementing the diet with rumen-protected amino acids. Knowledge derived in research setting is transferred into the real world through several large on-farm projects in central and south-central Pennsylvania. Another area of research is focused on the use of plant-derived bioactive compounds to mitigate enteric methane production in dairy cows and enhance their immune function, improve nutrient utilization, gut and overall animal health, and increase milk production.